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KMID : 0364819720100030109
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Korean Journal of Microbiology
1972 Volume.10 No. 3 p.109 ~ p.116
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Studies on Production of Nucleic acid Derivatives by Microorganisms(II); Accumulation of 5´-Inosinic acid by adenineless Mutant of Brevibacterium ammoniagenes
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Abstract
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Three strains among 120 adenineless mutants of Brevibacterium ammoniagenes described in the previous paper were screened out to accumulate UV absorbing substances in the culture broth. It was analyzed to be 5´-inosinic acid and hypoxanthine by means of two dimensional paper chromatography, UV absorption spectra and periodate oxidation. 5´-IMP was isolated from the culture broth of the mutant No.203 with anion-exchange resin amberite IRA-400 and recrystallized from ethanol. It was proved identical to authentic sample by Infra-red absorption spectrum. The growth response of the mutant No.203 were demonstrated to require adenine, but not with adenosine and 5´-AMP.
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